Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.Mikhail A. Galkin 1, Aidan N. Russell 2, Steven B. Vik 3, Richard M. Berry 2, Robert R. Ishmukhametov 2
1First Pavlov State Medical University, 2Clarendon Laboratory, Department of Physics, Oxford University, 3Department of Biological Sciences, Southern Methodist University
Here we present two ultrafast protocols for reconstitution of membrane proteins into fusogenic proteoliposomes, and fusion of such proteoliposomes with target lipid bilayers for detergent-free delivery of these membrane proteins into the postfusion bilayer. The combination of these approaches enables fast and easily controlled assembly of complex multi-component membrane systems.
