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DZHK (German Center for Cardiovascular Research)

3 ARTICLES PUBLISHED IN JoVE

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Biology

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Julia U. Sprenger 1, Ruwan K. Perera 1, Konrad R. Götz 1, Viacheslav O. Nikolaev 1
1Emmy Noether Group of the DFG, Department of Cardiology and Pneumology, European Heart Research Insitute Göttingen, Georg August University Medical Center, Göttingen, Germany

Förster resonance energy transfer (FRET) microscopy is a powerful technique for real-time monitoring of signaling events in live cells using various biosensors as reporters. Here we describe how to build a customized epifluorescence FRET imaging system from commercially available components and how to use it for FRET experiments.

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Medicine

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System
Christiane Jungen 1,2, Katharina Scherschel 1,2, Nadja I. Bork 2,3, Pawel Kuklik 1, Christian Eickholt 1, Helge Kniep 1, Niklas Klatt 1,2, Stephan Willems 1,2, Viacheslav O. Nikolaev 2,3, Christian Meyer 1,2
1Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, 2DZHK (German Center for Cardiovascular Research), 3Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf

Here, we present a protocol for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cAMP dynamics using an ex vivo Langendorff setup.

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Medicine

Location, Dissection, and Analysis of the Murine Stellate Ganglion
Katharina Scherschel 1,2,3, Hanna Bräuninger 2,4, Klara Glufke 2, Christiane Jungen 2,4,5, Nikolaj Klöcker 3, Christian Meyer 1,2,3
1Division of Cardiology, EVK Düsseldorf, cNEP, cardiac Neuro- and Electrophysiology Research Consortium, 2DZHK (German Centre for Cardiovascular Research), 3Institute of Neural and Sensory Physiology, Medical Faculty, Heinrich Heine University Düsseldorf, 4Clinic for Cardiology, University Heart & Vascular Centre, University Hospital Hamburg-Eppendorf, 5Department of Cardiology, Leiden University Medical Center

Pathophysiological changes in the cardiac autonomic nervous system, especially in its sympathetic branch, contribute to the onset and maintenance of ventricular arrhythmias. In the present protocol, we show how to characterize murine stellate ganglia to improve the understanding of the underlying molecular and cellular processes.

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