Purification and Reconstitution of TRPV1 for Spectroscopic AnalysisFrancisco J. Sierra-Valdez 1, Richard A. Stein 2, Phanindra Velissety 1,3, Valeria Vasquez 1, Julio F. Cordero-Morales 1
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 3CuriRX, Inc.
This article describes specific methods to obtain biochemical quantities of detergent-solubilized TRPV1 for spectroscopic analysis. The combined protocols provide biochemical and biophysical tools that can be adapted to facilitate structural and functional studies for mammalian ion channels in a membrane-controlled environment.
