Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse RetinaRatnesh K. Singh 1, Pablo E. Diaz 1, François Binette 1, Igor O. Nasonkin 1
1BioTime, Inc
The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.