Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow CytometryMaria Letizia Giardino Torchia 1, Raffaello Cimbro 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine
Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.
