Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass SpectrometryAmi Maruyama 1,2, Kenjiro Kami 3, Kazunori Sasaki 3, Hajime Sato 3, Yuzo Sato 1,2, Katsuya Tsuchihara 4, Hideki Makinoshima 1,2,4
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory, National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center
The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.
