Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible MethodsJiajun Yap 1, Jimin Yuan 1, Zi Heng Tee 1, Xuchao Huang 1, Wan Hwa Ng 1, Jiancheng Hu 1,2
1The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research, National Cancer Centre Singapore, 2The Cancer and Stem Cell Program, Duke-NUS Medical School
In this article, we presented a set of practical and feasible methods for characterizing disease-related mutants of RAF family kinases, which include in vitro kinase assay, RAF co-activation assay, and complementary split luciferase assay.
