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Ministry of Health and Welfare

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Yu-Jen Chen^1,2, Hui-Yu Liu², Yun-Tsui Chang², Ying-Hung Cheng², Harry J. Mersmann², Wen-Hung Kuo³, Shih-Torng Ding^1,2

¹Institute of Biotechnology, National Taiwan University, ²Department of Animal Science and Technology, National Taiwan University, ³Department of Surgery, National Taiwan University Hospital and College of Medicine

This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

Medicine

Integration of Brain Tissue Saturation Monitoring in Cardiopulmonary Exercise Testing in Patients with Heart Failure

Shu-Chun Huang^1,2,3, Carl PC Chen^1,3, Tieh-Cheng Fu⁴, Yu-Jen Chen^1,5,6

¹Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, ²Healthy Aging Research Center, Chang Gung University, ³School of Medicine, College of Medicine, Chang Gung University, ⁴Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Keelung, ⁵National Quemoy University, ⁶Kinmen Hospital, Ministry of Health and Welfare

This protocol integrated near-infrared spectroscopy into conventional cardiopulmonary exercise testing to identify the involvement of the cerebral hemodynamic response in exercise intolerance in patients with heart failure.