3D Particle Tracking for Noninvasive In Vivo Analysis of Synaptic Microtubule Dynamics in Dendrites and Neuromuscular Junctions of DrosophilaVivian T. Chou *1, Hunkar Gizem Yesilyurt *1, Hoyin Lai *2, Jennifer B. Long 1, Mercedes Arnes 1, Kamal Obbad 1, Michael Jones 2, Hideki Sasaki 2, Luciano A.G. Lucas 2, Sam Alworth 2, James Shih-Jong Lee 2, David Van Vactor 1
1Blavatnik Institute of Cell Biology and Program in Neuroscience, Harvard Medical School, 2DRVision Technologies LLC
This study presents a noninvasive intravital neuronal imaging strategy combined with a new software strategy to achieve automated, unbiased tracking and analysis of in vivo microtubule (MT) plus-end dynamics in the sensory dendrites and the neuromuscular junctions of Drosophila.
