Methylated RNA Immunoprecipitation Assay to Study m5C Modification in ArabidopsisEleftheria Saplaoura *1, Valentina Perrera *2, Vincent Colot 3, Friedrich Kragler 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
The methylated RNA immunoprecipitation assay is an antibody-based method used to enrich for methylated RNA fragments. Coupled with deep sequencing, it leads to the identification of transcripts carrying the m5C modification.
