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Sorbonne Université, CNRS

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Frédéric Catez^1,2,5, Antoine Rousseau⁴, Marc Labetoulle⁴, Patrick Lomonte^1,2,3

¹Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire, CNRS UMR 5534, ²Université de Lyon 1, ³Laboratoire d'excellence, LabEX DEVweCAN, ⁴Institut de Virologie Moléculaire et Structurale, CNRS UPR 3296, ⁵Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR 5286

We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.

JoVE Core

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft

Océane Blaise^*1,2, Constance Duchesne^*1,2, Sébastien Banzet², Antoine Rousseau¹, Nadira Frescaline^1,2

¹Laboratoire de Physique des Plasmas, École Polytechnique, Sorbonne Université, CNRS, ²Institut de Recherche Biomédicale des Armées, Clamart, INSERM UMRS-MD

The aim of this study was to develop a murine model of burn wound healing. A thermal burn was induced on the dorsal skin of mice using a preheated brass template. Burned tissue was debrided and overlaid with a skin graft harvested from the tail of a genetically similar donor mouse.