Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial CystsLatifa Bouzhir *1, Emilie Gontran *1, Lorena Loarca 1, Mauricette Collado-Hilly 1, Pascale Dupuis-Williams 1,2
1Physiopathogénèse et traitement des maladies du foie, Université Paris-Saclay, Inserm U1193, 2ESPCI Paris, Université PSL
Three-dimensional (3D) cellular systems are relevant models for investigating organogenesis. A hydrogel-based method for biliary cysts production and their characterization is proposed. This protocol unravels the barriers of 3D characterization, with a straightforward and reliable method to assess cyst formation efficiency, sizes, and to test their functionality.
