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AbbVie, Cambridge Research Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

Rui Wang¹, Zi-tong Huang³, Wen-kang Ren¹, Jiao Zhang¹, Yao Zhang¹, Bo Tan³, Ping Huang^1,2, Hong-ying Cao^1,2

¹School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, ²Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese Medicine, ³School of Basic Medical Sciences, Guangzhou University of Chinese Medicine

This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments.

Immunology and Infection

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Sean Graham¹, Lei Gao¹, Rui Wang¹

¹AbbVie, Cambridge Research Center

Here, we describe the methodology to knock out a gene of interest in the immune system using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease (Cas9)-based technologies and the evaluation of these mice in a cluster of differentiation 40 (CD40) agonistic antibody-induced colitis model.