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Yangsan Pusan National University Hospital

1 ARTICLES PUBLISHED IN JoVE

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Medicine

Preparation of Decellularized Kidney Scaffolds in Rats
Eun Heui Kim 1, Sang Soo Kim 1, Ju In Kim 2, Ji Min Cheon 2, Joo Hyoung Kim 3, Jin Chun Lee 4, Soo Geun Wang 5, Kyung Un Choi 6
1Department of Internal Medicine and Biomedical Research Institute, Pusan National University Hospital, 2Biomedical Research Institute, Pusan National University Hospital, 3Department of Plastic Surgery, Pusan National University Hospital, 4Department of Otorhinolaryngology - Head and Neck Surgery, Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology - Head and Neck Surgery, Pusan National University Hospital, 6Department of Pathology, Pusan National University Hospital

This protocol introduces a method to develop a scaffold using decellularized rat kidneys. The protocol includes decellularization and recellularization processes to confirm bioavailability. Decellularization is performed using Triton X-100 and sodium dodecyl sulfate.

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