Co-Culturing Microglia and Cortical Neurons Differentiated from Human Induced Pluripotent Stem CellsKara Lopez-Lengowski 1,2, Annie Kathuria 1,2,3, Kaia Gerlovin 1,2, Rakesh Karmacharya 1,2,3,4,5,6,7
1Center for Genomic Medicine, Massachusetts General Hospital, 2Chemical Biology Program, Broad Institute of MIT & Harvard, 3Department of Psychiatry, Harvard Medical School, 4Schizophrenia and Bipolar Disorder Program, McLean Hospital, 5Program in Neuroscience, Harvard University, 6Program in Chemical Biology, Harvard University, 7Harvard Stem Cell Institute
This protocol describes a methodology to differentiate microglia from human iPSCs and maintain them in co-culture with iPSC-derived cortical neurons in order to study mechanistic underpinnings of neuroimmune interactions using human neurons and microglia.
