Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer CellsMalcolm Lim 1, Nicholas Fletcher 2, Amy McCart Reed 1, Melanie Flint 3, Kristofer Thurecht 2, Jodi Saunus *1,4, Sunil R. Lakhani *1,5
1UQ Centre for Clinical Research, The University of Queensland, 2Centre for Advanced Imaging, Australian Institute for Bioengineering and Nanotechnology and ARC Training Centre for Innovation in Biomedical Imaging Technology, The University of Queensland, 3School of Biomedical Sciences, The University of Queensland, 4Mater Research and Mater Research Institute, The University of Queensland, 5Pathology Queensland, Royal Brisbane Women's Hospital
Brain metastasis is a cause of severe morbidity and mortality in cancer patients. Most brain metastasis mouse models are complicated by systemic metastases confounding analysis of mortality and therapeutic intervention outcomes. Presented here is a protocol for internal carotid injection of cancer cells that produces consistent intracranial tumors with minimal systemic tumors.
