Entrar

Key Laboratory of Ethnomedicine (Minzu University of China)

3 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
Qiao Li 1, Lin Lu 1, Huimin Tao 1, Carolyn Xue 1, Seagal Teitz-Tennenbaum 2, John H. Owen 3, Jeffrey S Moyer 3, Mark E.P. Prince 3, Alfred E. Chang 1, Max S. Wicha 2
1Department of Surgery, University of Michigan, 2Department of Internal Medicine, University of Michigan, 3Department of Otolaryngology, University of Michigan

Evaluated in syngeneic immunocompetent hosts, cancer stem cell (CSC) based dendritic cell (DC) vaccine demonstrated significantly higher antitumor immunity than traditional DC vaccines pulsed with heterogeneous bulk tumor cells.

image

Biology

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
Chang Li *1,2, Zhu Hui Liu *1,2, Jia Wei Chen 1,2, Xin Yi Shu 1,2, Ying Shen 1, Feng Hua Ding 1, Rui Yan Zhang 1, Wei Feng Shen 1, Lin Lu 1, Xiao Qun Wang 1
1Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 2Institute of Cardiovascular Diseases, Shanghai Jiao Tong University

Here, we present a protocol for immunofluorescence staining to observe the endothelial cells of the mouse aorta directly. This technique is useful when studying the cellular and molecular phenotype of endothelial cells in different flow patterns and in the development of atherosclerosis.

image

Bioengineering

Co-Fermentation of Marsdenia tenacissima with Ganoderma lucidum and Anti-Lung Cancer Effect of the Fermentation Products
Runtian Li 1,2, Zhiguang Zhang 1,2, Siqi Wang 1,2, Jiaoneng Yu 1,2, Xinxin Su 1,2, Lin Lu 1,2, Tongxiang Liu 1,2
1School of Pharmacy, Minzu University of China, 2Ministry of Education, Key Laboratory of Ethnomedicine (Minzu University of China)

Here, we describe a protocol to establish a systematic and comprehensive co-fermentation system to produce biotransformation products rich in pungent saponins by using Marsdenia tenacissima (Roxb.) Wight et Arn. (MT) as a fermentation medium for Ganoderma lucidum. This will serve as a methodological reference for the development of other ethnic drugs.

JoVE Logo

Privacidade

Termos de uso

Políticas

Pesquisa

Educação

SOBRE A JoVE

Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados