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Lallemand Animal Nutrition

1 ARTICLES PUBLISHED IN JoVE

Biology

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Eloïse Mussard^1,2, Corinne Lencina¹, Gaëlle Boudry³, Caroline S. Achard², Christian Klotz⁴, Sylvie Combes¹, Martin Beaumont¹

¹GenPhySE, Université de Toulouse, INRAE, ENVT, ²Lallemand Animal Nutrition, ³Institut NuMeCan, INRAE, INSERM, Université de Rennes, ⁴FG 16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute

Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.