Estimation of Total Lipid Volume from Decellularized Muscles by Lipid Extraction

Begin by transferring the decellularized skeletal muscles stained with ORO to 200 microliters of isopropanol in the individual wells of a 96 well plate. Agitate the solution by tapping the plate. And mechanically crush the muscle with a pipette tip under a microscope until no red spheres can be seen in the decellularized muscle suspension.

Again, mix the cell suspension in each well by pipetting up and down before transferring 75 microliters to two clean wells of the plate. Cover the plate and read the absorbance of the duplicate 75 microliter wells using a spectrophotometer or a plate reader.