Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

To begin, prepare 10 milliliters of 2X AlkB reaction buffer and sterilize through a 0.2 micron syringe filter. In a sterile PCR tube, add 20 microliters of the linker 1-ligated RNAs, 50 microliters of the 2X AlkB reaction buffer, two microliters o