Preparing the 96.96 Dynamic Array IFC, the Samples, and the 13x Assays
5:25
qRT-PCR Analysis of miRNA Expression in Mouse ESC and Human Serum
6:10
qRT-PCR Analysis
7:04
Conclusion
Here we describe an optimized multiplex reverse transcriptase quantitative PCR (qRT-PCR) protocol in combination with a microfluidic platform as a cost and time effective high-throughput screening tool for microRNA (miRNA) expression levels, especially when working with limited amounts of sample.