Evaluation of Insulin Action in Chow-fed vs. High Fat-fed Obese Mice using the Hyperinsulinemic-euglycemic Clamp
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Conclusion
The hyperinsulinemic-euglycemic clamp, or insulin clamp, is the gold standard for assessing insulin action in vivo. A method for performing insulin clamps in mice is described. This includes a method for arterial catheterization that permits experiments to be performed in conscious, unrestrained mice with minimal stress.