Name: renal capsule xenografting and subcutaneous pellet implantation for the evaluation of prostate carcinogenesis and benign prostatic hyperplasia
Uploaded: 2013-08-28T14:45:00
Description: Watch this Scientific Journal Video about Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia at JoVE.com

The authors would like to thank all members of the Ricke lab, past and present. We thank Calvin Patten, Jr., DVM and Brigitte Raabe, DVM, for helpful comments regarding the protocol. We would like to acknowledge the NIDDK, NCI, and NIEHS for their financial support for these studies: R01DK093690, R01CA123199, RC2ESO18764. TMN is a trainee in the Medical Scientist Training Program at the University of Rochester funded by NIH T32 GM07356; the NIH under Ruth L. Kirschstein National Research Service Award F30DK093173 also supports this project. CDV is supported by T32CA157322 and ABT is supported by T32ES007015 at the University of Wisconsin-Madison. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or NIH.

1. Preparation of Compressed Hormone/Drug Pellet
<ol>
	<li>This procedure should be done in a chemical safety hood while wearing a laboratory coat, gloves, mask, safety glasses, and bonnet. To prevent cross-contamination, it is critical that the pellet making equipment is thoroughly cleaned with 70% ethanol prior to and after manufacture of each type of pellet.</li>
	<li>Using an analytical scale and weigh paper, measure desired amount of hormone/drug powder. Approximately 5% extra material is recommended to accommodat...

<ol>
	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Cancer Facts Figures. 4, (2010).</li><li>McVary, K. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=BPH:+epidemiology+and+comorbidities.">BPH: epidemiology and comorbidities.</a> Am. J. Manag. Care. 12, S122-S128 (2006).</li><li>Nicholson, T. M., Ricke, W. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Androgens+and+estrogens+in+benign+prostatic+hyperplasia:+past,+present+and+future.">Androgens and estrogens in benign prostatic hyperplasia: past, present and future.</a> Differentiation. 82, 184-199 (2011).</li><li>Ricke, W. A., Wang, Y., Cunha, G. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steroid+hormones+and+carcinogenesis+of+the+prostate:+the+role+of+estrogens.">Steroid hormones and carcinogenesis of the prostate: the role of estrogens.</a> Differentiation. 75, 871-882 (2007).</li><li>Ricke, W. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steroid+hormones+stimulate+human+prostate+cancer+progression+and+metastasis.">Steroid hormones stimulate human prostate cancer progression and metastasis.</a> Int. J. Cancer. 118, 2123-2131 (2006).</li><li>Belanger, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+serum+concentrations+of+conjugated+and+unconjugated+steroids+in+40-+to+80-year-old+men.">Changes in serum concentrations of conjugated and unconjugated steroids in 40- to 80-year-old men.</a> J. Clin. Endocrinol. 79, 1086-1090 (1994).</li><li>Nicholson, T. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Testosterone+and+17beta-estradiol+induce+glandular+prostatic+growth,+bladder+outlet+obstruction,+and+voiding+dysfunction+in+male+mice.">Testosterone and 17beta-estradiol induce glandular prostatic growth, bladder outlet obstruction, and voiding dysfunction in male mice.</a> Endocrinology. 153, 5556-5565 (2012).</li><li>Str&#246;m, J. O., Theodorsson, A., Ingberg, E., Isaksson, I. M., Theodorsson, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ovariectomy+and+17&#946;-estradiol+Replacement+in+Rats+and+Mice:+A+Visual+Demonstration.">Ovariectomy and 17&#946;-estradiol Replacement in Rats and Mice: A Visual Demonstration.</a> J. Vis. Exp. (64), e4013 (2012).</li><li>Szot, G. L., Koudria, P., Bluestone, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+Pancreatic+Islets+Into+the+Kidney+Capsule+of+Diabetic+Mice.">Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice.</a> J. Vis. Exp. (9), e404 (2007).</li><li>Aamdal, S., Fodstad, O., Nesland, J. M., Pihl, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characteristics+of+human+tumour+xenografts+transplanted+under+the+renal+capsule+of+immunocompetent+mice.">Characteristics of human tumour xenografts transplanted under the renal capsule of immunocompetent mice.</a> Brit. J. Cancer. 51, 347-356 (1985).</li><li>Cunha, G. R., Lung, B., Reese, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glandular+epithelial+induction+by+embryonic+mesenchyme+in+adult+bladder+epithelium+of+BALB/c+mice.">Glandular epithelial induction by embryonic mesenchyme in adult bladder epithelium of BALB/c mice.</a> Investigative urology. 17, 302-304 (1980).</li><li>Norman, J. T., Cunha, G. R., Sugimura, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+induction+of+new+ductal+growth+in+adult+prostatic+epithelium+in+response+to+an+embryonic+prostatic+inductor.">The induction of new ductal growth in adult prostatic epithelium in response to an embryonic prostatic inductor.</a> The Prostate. 8, 209-220 (1986).</li><li>Hayward, S. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interactions+between+adult+human+prostatic+epithelium+and+rat+urogenital+sinus+mesenchyme+in+a+tissue+recombination+model.">Interactions between adult human prostatic epithelium and rat urogenital sinus mesenchyme in a tissue recombination model.</a> Differentiation. 63, 131-140 (1998).</li><li>Wang, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+human+prostatic+epithelial+model+of+hormonal+carcinogenesis.">A human prostatic epithelial model of hormonal carcinogenesis.</a> Cancer Res. 61, 6064-6072 (2001).</li><li>Ricke, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Androgen+hormone+action+in+prostatic+carcinogenesis:+stromal+androgen+receptors+mediate+prostate+cancer+progression,+malignant+transformation+and+metastasis.">Androgen hormone action in prostatic carcinogenesis: stromal androgen receptors mediate prostate cancer progression, malignant transformation and metastasis.</a> Carcinogenesis. 33, 1391-1398 (2012).</li><li>Wang, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+and+characterization+of+efficient+xenograft+models+for+benign+and+malignant+human+prostate+tissue.">Development and characterization of efficient xenograft models for benign and malignant human prostate tissue.</a> The Prostate. 64, 149-159 (2005).</li><li>Goto, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proximal+prostatic+stem+cells+are+programmed+to+regenerate+a+proximal-distal+ductal+axis.">Proximal prostatic stem cells are programmed to regenerate a proximal-distal ductal axis.</a> Stem Cells. 24, 1859-1868 (2006).</li><li>Xin, L., Ide, H., Kim, Y., Dubey, P., Witte, O. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+regeneration+of+murine+prostate+from+dissociated+cell+populations+of+postnatal+epithelia+and+urogenital+sinus+mesenchyme.">In vivo regeneration of murine prostate from dissociated cell populations of postnatal epithelia and urogenital sinus mesenchyme.</a> Proc. Natl. Acad. Sci. U.S.A. 100, 11896-11903 (2003).</li><li>Priolo, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishment+and+genomic+characterization+of+mouse+xenografts+of+human+primary+prostate+tumors.">Establishment and genomic characterization of mouse xenografts of human primary prostate tumors.</a> Am. J. Pathol. 176, 1901-1913 (2010).</li><li>Pritchett-Corning, K. R., Mulder, G. B., Luo, Y., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+Rodent+Surgery+for+the+New+Surgeon.">Principles of Rodent Surgery for the New Surgeon.</a> J. Vis. Exp. (47), e2586 (2011).</li></ol>

This paper and the protocol outlined herein describe the manufacture of compressed hormone and/or drug pellets, and the surgical procedure for subcutaneous pellet implantation of mice. Depending on the research question to be addressed, this technique can be performed singly, or in combination with renal capsule xenografting of prostate cell recombinant grafts or prostate tissue xenografts, which are techniques widely used in prostate research 17-19. Taken together, these techniques offer a powerful approach t...

Prostate cancer (PrCa) and benign prostatic hyperplasia (BPH) are significant health burdens. PrCa is the second most prevalent solid organ cancer in men and a leading cause of cancer related death 1. BPH is also highly prevalent among older men, and it is estimated that the clinical manifestation of BPH, lower urinary tract symptoms (LUTS), will affect 50-90% of men 2. While androgens and estrogens are known to be important in PrCa and BPH, our understanding of the hormonal mechanisms that underlie carcinogenesis and growth remains incomplete 3,4. Simple and genetically tractable animal models underpin the studies that will address these priorities in prostate research. In hormone responsive diseases such as PrCa and BPH, the use of subcutaneous, slow release hormone pellets, in isolation or in combination with renal capsule xenografting (the transfer of cells, tissues or organs from one species into another) into the immunocompromised mouse host, provides a simple and reproducible method for studying hormonal regulation of growth and carcinogenesis.
Subcutaneous hormone pellet implantation is a simple and reproducible technique to study hormonal regulation of carcinogenesis and benign growth in the prostate 5. Subcutaneous implantation of adult male mice with 25 mg testosterone (T) and 2.5 mg 17&beta;-estradiol (E2), causes an increase in serum E2 and gradual decrease in T, recreating the dynamic hormonal environment of aging men 5,6. In addition, this model recapitulates many of the clinical features of BPH-LUTS 5,7. Alternative methods of hormone administration include oral/gavage administration or intraperitoneal injection, which cause distress to the rodent, are less consistent in delivering the same amount of drug over time, and are more labor-intensive than a one-time surgical implantation. Other subcutaneous modes for hormone and/or drug delivery include Silastic capsules 8. While we also have experience with use of Silastic capsules, compressed pellets are favored for their ease of use and reproducibility. Furthermore, release rates of compressed hormone pellets better mimic the dynamic sex steroid hormone ratios observed in aging men 5,7.
Since the development of genetically immunocompromised mice, numerous in vivo model systems incorporating xenografting techniques have been developed for the study of a wide variety of normal and diseased tissues. There are several basic categories of xenografting. Tissue xenografts consist of a small piece of intact tissue, which can be a normal structure, malignant tumor, or benign growth. Szot et al. (2007) have recently illuminated renal capsule xenografting of pancreatic islets 9. Aamdal et al. (1985) described renal capsule xenografting of 27 human cancer cell lines in immunocompromised mouse hosts 10. Grafts can also be composed of a single immortalized cell line (cancerous or non-tumorigenic), or can consist of an immortalized cell line combined with cells isolated from mesenchyme (cell recombinant graft).
We favor xenografting of cell recombinants to probe the respective roles of stroma and epithelium in the development of PrCa and BPH. Cunha et al. (1980) was the first to report that when adult murine bladder epithelium is combined with embryonic urogenital sinus mesenchyme (UGM) and xenografted under the renal capsule of male mice hosts, the tissue develops into structures resembling prostate acini 11. Norman et al. (1986) showed that adult mouse prostatic ductal epithelium and UGM, when combined in tissue recombinants, undergo ductal growth and branching morphogenesis 12. Hayward et al. (1988) showed that human prostate epithelium responds to inductive fetal mesenchyme in a similar fashion 13. A hormonal model of carcinogenesis in the prostate, by combining the immortalized human prostate epithelial cell line BPH-1 with UGM, was first reported by Wang et al. 14 (2001) and has been used extensively in our laboratory 15. This model is well suited for understanding PrCa progression because benign prostatic epithelium transforms to metastatic disease, modeling advanced PrCa in humans 5. Because specific components can be genetically manipulated, cell recombinant grafting is particularly useful as an approach to evaluate stromal-epithelial interactions.
Other sites suitable for xenografting are intradermal, subcutaneous and orthotopic locations 16. Depending on the tissue and disease process of interest, these may be suitable alternative approaches. For the study of prostate hormonal carcinogenesis and benign growth regulation, we choose the renal capsule site for xenografting due to its higher graft take rate, abundant blood supply, and ability to implant a greater number of xenografts into one confined site 16. Moreover, hormonal manipulation of the host mouse that results in prostatic atrophy limits the use of prostate orthotopic grafting 16.
This protocol outlines techniques of compressed hormone/drug pellet manufacture and surgical implantation, as well as renal capsule xenografting of human prostate cell and tissue grafts. Together, these techniques provide a sensitive assay to determine if a genetically modified mouse is more susceptible to PrCa and/or BPH initiation than control strains. Xenografting is a powerful technique for in vivo evaluation of the potential of experimental cells to develop histologic and molecular features of malignancy, as well as an assay for novel treatment strategies for a wide variety of benign and malignant diseases.

<table border="1">
		<tbody>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>EQUIPMENT &amp; MATERIALS</td>
		 </tr>
		 <tr>
			<td>Pellet press</td>
			<td>PARR Industries </td>
			<td>2815</td>
			<td>3mm Punch &amp; Die set</td>
		 </tr>
		 <tr>
			<td>Analytical Balance</td>
			<td>Ohaus</td>
			<td>1918302</td>
			<td>Voyager or other</td>
		 </tr>
		 <tr>
			<td>Bunsen burner</td>
			<td>Fisher Scientific</td>
			<td>03-962Q</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dissecting microscope</td>
			<td>Leica</td>
			<td>L2</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Deltaphase Pad Kit (reheatable wax)</td>
			<td>Braintree Scientific</td>
			<td>39DP</td>
			<td>Alternative to an electric heating pad</td>
		 </tr>
		 <tr>
			<td>Hot Bead sterilizer</td>
			<td>F.S.T.</td>
			<td>18000-45</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Isoflurane Vaporizer</td>
			<td>Supera Anesthesia Innov</td>
			<td>VAP3000</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Induction Chamber</td>
			<td>Supera Anesthesia Innov</td>
			<td>RES644</td>
			<td></td>
		 </tr>
		 <tr>
			<td>F/AIR Canister</td>
			<td>Supera Anesthesia Innov</td>
			<td>80120</td>
			<td></td>
		 </tr>
		 <tr>
			<td>4 port Manifold</td>
			<td>Supera Anesthesia Innov</td>
			<td>RES536</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Rebreathing Circuits</td>
			<td>Supera Anesthesia Innov</td>
			<td>CIR529</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Inlet/Outlet Fittings</td>
			<td>Supera Anesthesia Innov</td>
			<td>VAP203/4</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Pressure Reg/Gauge</td>
			<td>Supera Anesthesia Innov</td>
			<td>OXY508</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Oxygen Flowmeter</td>
			<td>Supera Anesthesia Innov</td>
			<td>OXY660</td>
			<td></td>
		 </tr>
		 <tr>
			<td>21mm Clear Tubing</td>
			<td>Supera Anesthesia Innov</td>
			<td>301-150</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Small Mice Nose Cone</td>
			<td>Supera Anesthesia Innov</td>
			<td>ACC526</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sterile surgical gown</td>
			<td>Midwest Vet Supply</td>
			<td>350.79866.2</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Surgical mask</td>
			<td>Midwest Vet Supply</td>
			<td>350.50111.2</td>
			<td>2-ply w/ earloops</td>
		 </tr>
		 <tr>
			<td>Sterile gauze</td>
			<td>Midwest Vet Supply</td>
			<td>001.14100.2</td>
			<td>2 x 2 inches</td>
		 </tr>
		 <tr>
			<td>Sterile Gloves</td>
			<td>Kimberly Clark</td>
			<td>55092</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Bouffant</td>
			<td>Midwest Vet Supply</td>
			<td>001.27100.2</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Cotton Tip Applicator</td>
			<td>Midwest Vet Supply</td>
			<td>001.06220.2</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Surgical Scrub Wash</td>
			<td>Midwest Vet Supply</td>
			<td>733.80010.3</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sterile Fields (Fenestrated)</td>
			<td>General Econopak, Inc</td>
			<td>88VCSTF</td>
			<td></td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>REAGENTS</td>
		 </tr>
		 <tr>
			<td>Cholesterol</td>
			<td>Sigma-Aldrich</td>
			<td>C-3292</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Testosterone Proprionate</td>
			<td>Sigma-Aldrich</td>
			<td>T-1875</td>
			<td></td>
		 </tr>
		 <tr>
			<td>17&beta;-estradiol</td>
			<td>Sigma-Aldrich</td>
			<td>E-2758</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Isoflurane</td>
			<td>Midwest Vet Supply</td>
			<td>193.33161.3</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Carprofen</td>
			<td>Midwest Vet Supply</td>
			<td>193.70200.3</td>
			<td>Injectable (Rimadyl)</td>
		 </tr>
		 <tr>
			<td>Betadine</td>
			<td>Webster Veterinary</td>
			<td>07-836-3379</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Sterile saline</td>
			<td>Midwest Vet Supply</td>
			<td>193.74504.3</td>
			<td>NaCl 0.9%, Injectable</td>
		 </tr>
		 <tr>
			<td></td>
			<td></td>
			<td></td>
			<td>SURGICAL INSTRUMENTS</td>
		 </tr>
		 <tr>
			<td>Straight Sharp/Blunt Scissors</td>
			<td>Fine Scientific Tools (F.S.T.)</td>
			<td>14054-13</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Graefe forceps (Serrated, Toothed, Curved)</td>
			<td>F.S.T.</td>
			<td>11055-10</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Fine Iris scissors</td>
			<td>F.S.T.</td>
			<td>14090-09</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Graefe Scalpel</td>
			<td>F.S.T.</td>
			<td>10071-12</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Dumont #5 forceps</td>
			<td>F.S.T.</td>
			<td>11251-20</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Glass Pasteur pipets</td>
			<td>Fisher Scientific</td>
			<td>S67050</td>
			<td>5" length</td>
		 </tr>
		 <tr>
			<td>Graefe forceps (Serrated, Curved)</td>
			<td>F.S.T.</td>
			<td>11052-10</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Graefe forceps (Serrated, Straight)</td>
			<td>F.S.T.</td>
			<td>11050-10</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Vicryl Suture</td>
			<td>Midwest Vet Supply</td>
			<td>295-92100.2</td>
			<td>4-0, FS-2, Absorbable</td>
		 </tr>
		 <tr>
			<td>Needle Holder w/ scissor action</td>
			<td>F.S.T.</td>
			<td>12002012</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Wound Clips</td>
			<td>Braintree Scientific</td>
			<td>ACS CS</td>
			<td>May use 7mm or 9mm clips</td>
		 </tr>
		 <tr>
			<td>Reflex Wound Clipper</td>
			<td>F.S.T.</td>
			<td>12031-09</td>
			<td>Correspond w/ wound clip size</td>
		 </tr>
		 <tr>
			<td>Would Clip Remover</td>
			<td>F.S.T.</td>
			<td>12033-00</td>
			<td>Universal</td>
		 </tr>
		 <tr>
			<td>Sterilization Container</td>
			<td>F.S.T.</td>
			<td>20810-01</td>
			<td>Any autoclavable container will do</td>
		 </tr>
		 <tr>
			<td>Syringe w/ needle</td>
			<td>BD</td>
			<td>148292C</td>
			<td>1cc, 25Gx1</td>
		 </tr>
		 <tr>
			<td>Ear Tag Applicator</td>
			<td>National Band &amp; Tag Co.</td>
			<td>1005s1</td>
			<td>Alternative to ear notching</td>
		 </tr>
		 <tr>
			<td>Small Animal Ear Tags</td>
			<td>National Band &amp; Tag Co.</td>
			<td>1005-1</td>
			<td></td>
		 </tr>
		</tbody>
 </table>

renal capsule xenografting and subcutaneous pellet implantation for the evaluation of prostate carcinogenesis and benign prostatic hyperplasia

New therapies for two common prostate diseases, prostate cancer (PrCa) and benign prostatic hyperplasia (BPH), depend critically on experiments evaluating their hormonal regulation. Sex steroid hormones (notably androgens and estrogens) are important in PrCa and BPH; we probe their respective roles in inducing prostate growth and carcinogenesis in mice with experiments using compressed hormone pellets. Hormone and/or drug pellets are easily manufactured with a pellet press, and surgically implanted into the subcutaneous tissue of the male mouse host. We also describe a protocol for the evaluation of hormonal carcinogenesis by combining subcutaneous hormone pellet implantation with xenografting of prostate cell recombinants under the renal capsule of immunocompromised mice. Moreover, subcutaneous hormone pellet implantation, in combination with renal capsule xenografting of BPH tissue, is useful to better understand hormonal regulation of benign prostate growth, and to test new therapies targeting sex steroid hormone pathways.

Depending on the hypothesis to be tested, renal capsule xenografting of tissue or cell recombinants can be performed in isolation, or in combination with subcutaneous hormone pellet implantation. A schematic of a hypothetical experiment combining renal capsule xenografting experiment with subcutaneous hormone pellet implantation is illustrated in Figure 1. A variety of powdered and/or crystalline substances can be used in the manufacture of compressed pellets with the pellet press. Figure 2</stro...

Watch this Scientific Journal Video about Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia at JoVE.com

Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia

We describe the manufacture of compressed hormone pellets, as well as subcutaneous surgical implantation into mice. This strategy can be combined with the growth of cell and tissue xenografts under the renal capsule of athymic nude mice to evaluate hormonal carcinogenesis and regulation of benign prostate growth.

New therapies for two common prostate diseases,  prostate cancer (PrCa) and benign prostatic hyperplasia (BPH), depend  critically on experiments ...

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Medicine

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Surgical Treatment for Benign Prostatic Hyperplasia: Holmium Laser Enucleation of the Prostate (HoLEP).

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