Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

DOI :

10.3791/51414-v

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June 9th, 2014

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14,199 Views

Bledar Bisha¹, Jaclyn A. Adkins², Jana C. Jokerst³, Jeffrey C. Chandler¹, Alma Pérez-Méndez⁴, Shannon M. Coleman⁴, Adrian O. Sbodio⁵, Trevor V. Suslow⁵, Michelle D. Danyluk⁶, Charles S. Henry², Lawrence D. Goodridge⁷

¹Department of Animal Science, University of Wyoming, ²Department of Chemistry, Colorado State University, ³Department of Environmental and Radiological Health Sciences, Colorado State University, ⁴Department of Animal Sciences, Colorado State University, ⁵Department of Plant Sciences, University of California, Davis, ⁶Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, ⁷Department of Food Science and Agricultural Chemistry, McGill University

Chapters

A protocol involving integrated concentration, enrichment, and end-point colorimetric detection of foodborne pathogens in large volumes of agricultural water is presented here. Water is filtered through Modified Moore Swabs (MMS), enriched with selective or non-selective media, and detection is performed using paper-based analytical devices (µPAD) imbedded with bacterial-indicative colorimetric substrates.

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