November 28th, 2015
•Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.
Tags
Vídeos Relacionados
Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Bone Marrow-derived Macrophage Production
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
Production and Characterization of Human Macrophages from Pluripotent Stem Cells
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados