Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
2:33
Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
4:07
Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
6:11
Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
7:37
Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
8:57
Conclusion
Transcrição
The overall goal of this experimental procedure is to use a liquid crystal based nanoparticle as a delivery platform for the controlled delivery of a hydrophobic dicargo to the lipophilic portion of the plasma membrane of living mammalian cells. T
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A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living cells.