The overall goal of this procedure is a rapid and efficient method to dissect pupal wings of Drosophila suitable for immunodetections or PCR assays. The procedure described here was developed by the demand to find a fast and efficient method to ob
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The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).