<h2 class="jove_title"><a href="https://www.jove.com/video/52047">Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells</a></h2>
Ieng Lam Lei1, Lei Bu2, Zhong Wang1
1Cardiac Surgery, University of Michigan, 2Leon H Charney Division of Cardiology, New York University School of Medicine
In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.
<h2 class="jove_title"><a href="https://www.jove.com/video/52042">Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes</a></h2>
Estelle Hirsinger1, Jo&#227;o Emanuel Carvalho2, Christine Chevalier1,3, Georges Lutfalla5, Jean-Fran&#231;ois Nicolas1, Nadine Peyri&#233;ras4, Michael Schubert2
1D&#233;partement de Biologie du D&#233;veloppement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du D&#233;veloppement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universit&#233;s, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Canc&#233;rologie de Marseille, 4Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard, 5Unit&#233; de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Universit&#233; Montpellier II
We report here the robust and efficient expression of fluorescent proteins after mRNA injection into unfertilized oocytes of Branchiostoma lanceolatum. The development of the microinjection technique in this basal chordate will pave the way for far-reaching technical innovations in this emerging model system, including in vivo imaging and gene-specific manipulations.
<h2 class="jove_title"><a href="https://www.jove.com/video/52049"> Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle </a></h2>
Chibeza C. Agley1,2, Anthea M. Rowlerson1, Cristiana P. Velloso1, Norman L. Lazarus1, Stephen D. R. Harridge1
1Centre of Human and Aerospace Physiological Sciences, King&#8216;s College London, 2Wellcome Trust-Medical Research Council, Cambridge Stem Cell Institute
The main adherent cell types derived from human muscle are myogenic cells and fibroblasts. Here, cell populations are enriched using magnetic-activated cell sorting based on the CD56 antigen. Subsequent immunolabelling with specific antibodies and use of image analysis techniques allows quantification of cytoplasmic and nuclear characteristics in individual cells. 
<h2 class="jove_title"><a href="https://www.jove.com/video/52181">Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis</a></h2>
Elizabeth R. Zielins*1, Ruth Tevlin*1, Michael S. Hu1, Michael T. Chung1, Adrian McArdle1, Kevin J. Paik1, David Atashroo1, Christopher R. Duldulao1, Anna Luan1, Kshemendra Senarath-Yapa1, Graham G. Walmsley1, Taylor Wearda1, Michael T. Longaker1,3, Derrick C. Wan1
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 2Stem Cell and Biomaterials Engineering Laboratory, Department of Bioengineering, Stanford University School of Medicine, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University
The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

january 2015: this month in jove - introducing jove developmental biology

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/95">January 2015 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
JoVE has been revolutionizing scientific publishing since 2006, when we released our first video articles in <a href="http://www.jove.com/biology">JoVE Biology</a>. We've grown over the years-adding sections in <a href="http://www.jove.com/neuro">Neuroscience</a>, <a href="http://www.jove.com/i2">Immunology &amp; Infection</a>, <a href="http://www.jove.com/ctm">Clinical &amp; Translational Medicine</a>, <a href="http://www.jove.com/bioengineering">Bioengineering</a>, <a href="http://www.jove.com/ap">Applied Physics</a>, <a href="http://www.jove.com/behavior">Behavior</a>, <a href="http://www.jove.com/chem">Chemistry</a>, and <a href="http://www.jove.com/enviro">Environment</a>.
We are now pleased to introduce a new addition to the JoVE family: <a href="http://www.jove.com/devbio">JoVE Development</a>, which covers the entire field of developmental biology from the underlying genetic and epigenetic mechanisms to the growth and differentiation of single cells into organs and whole organisms.
In many species, developmental processes offer clues about evolution. For example, the European lancelet (Branchiostoma lanceolatum) has many features of modern fish, but it doesn't have a backbone-so it's emerging as a useful model for studying the divergence of vertebrates from invertebrate ancestors. <a href="http://www.jove.com/video/52042">Hirsinger et al.</a> have developed a method for visualizing embryonic development in the European lancelet. They inject oocytes with mRNAs that encode fluorescent proteins, and following fertilization, developmental processes can be visualized in vivo. 
Development also explores interactions between different cell types, and these interactions are fundamental for repairing and regenerating damaged or diseased tissues, like muscle. To study these interactions, <a href="http://www.jove.com/video/52049">Agley et al.</a> take human skeletal muscle biopsies then purify and culture different cell types. They also characterize the cells using immunocytochemical methods that can be adapted to other cell types.
One of the most exciting topics in developmental biology is the engineering of stem cells for experimental and clinical applications. This month we feature two stem cell protocols: <a href="http://www.jove.com/video/52181">Zielins et al.</a> describe the isolation and purification of human adipose-derived stromal cells for bone engineering, and <a href="http://www.jove.com/video/52047">Lei et al.</a> demonstrate how to differentiate embryonic stem cells into embryoid bodies, and then derive cardiac progenitor cells that further differentiate into cardiomyocytes and smooth muscle cells.
Above all, developmental processes are fascinating to visualize, and <a href="http://www.jove.com/devbio">JoVE Development</a> features techniques for optimizing real-time imaging, such as this protocol for live-imaging of the Drosophila pupal eye. Using image-stabilization techniques, <a href="http://www.jove.com/video/52120">Hellerman et al.</a> compensate for tissue movement and uneven topology to enhance the visualization of the developing Drosophila eye.
You've just had a sneak peek of JoVE's new <a href="http://www.jove.com/devbio">Development section</a> in the <a href="http://www.jove.com/archive/95">January 2015 issue</a>. Visit the website to see the full-length articles plus our other scientific sections in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about January 2015: This Month in JoVE - Introducing JoVE Developmental Biology at JoVE.com

January 2015: This Month in JoVE - Introducing JoVE Developmental Biology

Here's a look at what's coming up in the January 2015 issue of JoVE: The Journal of Visualized Experiments.
JoVE has been revolutionizing scientific ...

january-2015-this-month-in-jove-introducing-jove-developmental-biology

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2.4K visualizações.  Massachusetts Eye and Ear. January 2015: This Month in JoVE - Introducing JoVE Developmental Biology