June 15th, 2018
•This article describes a simple protein microarray method for profiling humoral immune responses to a 7-plex panel of highly purified Clostridium difficile antigens in human sera. The protocol can be extended for the determination of specific antibody responses in preparations of polyclonal intravenous immunoglobulin.
Tags
Vídeos Relacionados
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados