<h2 class="jove_title"><a href="https://www.jove.com/video/55287">Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays</a></h2>Syrena C. Fernandes, Daniel J. Wilson, Charles R. MaceDepartment of Chemistry, Tufts UniversityWe detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.<h2 class="jove_title"><a href="https://www.jove.com/video/55386">Absolute Quantification of A&#946;1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure</a></h2>Josef Pannee1,2, Kaj Blennow1,2, Henrik Zetterberg1,2,3, Erik Portelius1,21Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3UCL Institute of Neurology, Queen SquareA reference measurement procedure for the absolute quantification of A&#946;1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.<h2 class="jove_title"><a href="https://www.jove.com/video/54781">High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR</a></h2>Laura B. Goodman1, Renee R. Anderson1, Marcia Slater2, Elen Ortenberg2, Randall W. Renshaw1, Brittany D. Chilson1, Melissa A. Laverack1, John S. Beeby1, Edward J. Dubovi1, Amy L. Glaser11Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.<h2 class="jove_title"><a href="https://www.jove.com/video/54784">Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation</a></h2>Ke Ma, Dominique P. Lippelt, Bernhard HommelCognitive Psychology Unit, Leiden UniversityHere, we describe virtual-hand and virtual-face illusion paradigms that can be used to study body-related self-perception/-representation. They have already been used in various studies to demonstrate that, under specific conditions, a virtual hand or face can be incorporated into one&#39;s body representation, suggesting that body representations are rather flexible.

jove monthly highlights: march 2017

Here's a look at what's coming up in the March 2017 issue of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
As the start of spring brings new life, we begin the month investigating exciting new developments in embryo storage for in-vitro fertility treatment. In <a href="http://www.jove.com/devbio">JoVE Developmental Biology</a>, <a href="http://www.jove.com/video/54871/modified-microsecure-vitrification-safe-simple-highly-effective">our authors</a> present a modified technique for vitrification and preservation of human embryos. This aseptic technique uses all FDA-compliant materials including ionomeric resin embryo straws and sterile flexipettes, and allows fast, safe, and repeatable embryo storage independent of operator variation. In addition, with a greater than 95% embryo survival-rate the method contributes to excellent pregnancy outcomes. 
Paper cards...printers, it's all so 1990's, right? Well, our next video highlight might make you reconsider. In <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a> this month, <a href="http://www.jove.com/video/55287/fabrication-three-dimensional-paper-based-microfluidic-devices-for">our authors</a> reveal 3-dimensional paper microfluidic devices for immunoassays. By patterning paper with hydrophobic barriers, the transfer of fluids can be controlled and directed. Stacking multiple layers of patterned paper creates sophisticated 3-D microfluidic networks that can be utilized in cheap and simple bioanalytical assays, such as the detection of Human Chorionic Gonadotriphin, or hCG - the pregnancy hormone. So don't throw out those old Valentine's Day cards yet - upcycle!
In <a href="http://www.jove.com/med">JoVE Medicine</a> this month, <a href="http://www.jove.com/video/55386/absolute-quantification-a1-42-csf-using-mass-spectrometric-reference">our authors</a> reveal an accurate procedure for quantifying the plaque-forming amyloid-beta protein in cerebral spinal fluid to provide diagnoses in suspected Alzheimer's cases. Current diagnosis is mostly based on mental state and cognitive testing, with some studies beginning to examine amyloid-beta levels, but this is hampered by variability and reproducibility issues. Here, our authors use a combination of solid-phase extraction and liquid chromatography tandem mass spectrometry to quantify amyloid-beta levels to a high degree of accuracy, quantifying the deposition of the protein in plaques in the brain and enhancing the power for accurate diagnoses.
Our final video highlight this month comes from <a href="http://www.jove.com/behavior">JoVE Behavior</a>, and showcases a new technique to create human body illusions. Here, <a href="http://www.jove.com/video/54784/creating-virtual-hand-virtual-face-illusions-to-investigate-self">our authors</a> demonstrate a computer-based virtual hand and face that move in synchrony with the operator's action, creating an illusion that the model is an extension of the participant's own body. This is a useful tool in the study of self-representation, and is an advance over traditional studies requiring demonstrators to mimic actions on participants in time with actions performed on a physical model body part. Ah, just one small step until I realize my dream of becoming a super-fast blue hedgehog.
You've just had a sneak peek of the March 2017 issue of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about JoVE Monthly Highlights: March 2017 at JoVE.com

JoVE Monthly Highlights: March 2017

Here's a look at what's coming up in the March 2017 issue of JoVE: The Journal of Visualized Experiments.
As the start of spring brings new life, we begin ...

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2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Um ano em análise

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...