<h2 class="jove_title"><a href="https://www.jove.com/video/56254">Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics</a></h2>Pavel Mokrej&#353;1, Matou&#353; Hu&#357;&#357;a1, Jana Pavla&#269;kov&#225;2, Pavl&#237;na Egner21<a href='/institutions/EU-Europe/CZ-Czech-Republic/25988-Tomas-Bata-University-in-Zlin'>Department of Polymer Engineering, Faculty of Technology, Tomas Bata University in Zl&#237;n</a>, 2<a href='/institutions/EU-Europe/CZ-Czech-Republic/25988-Tomas-Bata-University-in-Zlin'>Department of Fat, Tenside and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zl&#237;n</a>The goal of the protocol is to prepare keratin hydrolysate from chicken feathers by alkaline-enzymatic hydrolysis and to test whether adding keratin hydrolysate into a cosmetics ointment base improves skin barrier function (heightening hydration and diminishing transepidermal water loss). Tests are conducted on men and woman volunteers.<h2 class="jove_title"><a href="https://www.jove.com/video/56641">Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics</a></h2>Kaiwen Sun*, Yuyu Zheng*, Ziqiang Zhu<a href='/institutions/AS-Asia/CN-China/26139-Nanjing-Normal-University'>Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University</a>This manuscript describes an easy and rapid experimental procedure for determining protein-protein interactions based on the measurement of luciferase activity.<h2 class="jove_title"><a href="https://www.jove.com/video/56591">Wind Tunnel Experiments to Study Chaparral Crown Fires</a></h2>Jeanette Cobian-I&#241;iguez1, AmirHessam Aminfar1, Joey Chong2, Gloria Burke2, Albertina Zuniga1, David R. Weise2, Marko Princevac11<a href='/institutions/NA-North-America/US-United-States/CA-California/14856-University-of-California,-Riverside'>Department of Mechanical Engineering, University of California, Riverside</a>, 2<a href='/institutions/NA-North-America/US-United-States/CA-California/26157-USDA-Forest-Service'>Pacific Southwest Research Station, USDA Forest Service</a>This protocol describes wind tunnel experiments designed to study the transition of a fire from the ground to the canopy of chaparral shrubs.<h2 class="jove_title"><a href="https://www.jove.com/video/56150">Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System</a></h2>Steven E. Naranjo1, Peter C. Ellsworth21<a href='/institutions/NA-North-America/US-United-States/AZ-Arizona/26083-USDA-ARS-Arid-Land-Agricultural-Research-Center'>USDA-ARS, Arid-Land Agricultural Research Center</a>, 2<a href='/institutions/NA-North-America/US-United-States/AZ-Arizona/26084-Maricopa-Agricultural-Center,-University-of-Arizona'>Department of Entomology, Maricopa Agricultural Center, University of Arizona</a>Life tables allow quantification of the sources and rates of mortality in insect populations and contribute to understanding, predicting and manipulating population dynamics in agroecosystems. Methods for conducting and analyzing cohort-based life tables in the field for an insect with sessile immature life stages are presented.

jove monthly highlights: november 2017

Here's a look at what's coming up in the November 2017 issue of JoVE: The World's Premier Video Journal.
From a cornucopia of articles for November, we begin with cosmetic applications for chicken feathers. In JoVE Biochemistry, our authors describe a method to prepare keratin hydrolysate from chicken feathers via alkaline-enzymatic hydrolysis, and demonstrate how to test its effect on the skin barrier. These keratin hydrolysate formulations improve skin barrier function by reducing transepidermal water loss.
Without "feather" ado, JoVE Environment goes from the farm to the field. Here, our authors present a methodology to create life tables of Bemisia tabaci, a global agricultural pest. Life tables allow quantification of the rates and sources of mortality in insect populations, and as these methods can be extrapolated to other pests, this research provides a valuable tool for the management of economically expensive pests in agroecosystems.
In JoVE Biology, we switch focus from the pests to the plants, with an experimental procedure for detecting protein-protein interaction dynamics in the tobacco plant Nicotiana benthemiana. A luciferase complementation assay is used to quantify the interaction between two proteins in leaves infiltrated with Agrobacterium tumefaciens. This method provides a simple, rapid proceedure that may be used to answer key questions in the plant signal transduction field.
Our final article, in JoVE Applied Physics, really turns up the heat. Here, our authors describe a series of wind tunnel experiments designed to study the transition of a fire from the ground to canopy in chaparral shrubs. Such experiments may lead to an increased understanding of chaparral crown fires, and can provide knowledge to aid in wildlife prediction and control. For that, we give thanks.
You've just had a sneak peek of the November 2017 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The World's Premier Video Journal.

Watch this Scientific Journal Video about JoVE Monthly Highlights: November 2017 at JoVE.com

JoVE Monthly Highlights: November 2017

Here's a look at what's coming up in the November 2017 issue of JoVE: The World's Premier Video Journal.
From a cornucopia of articles for November, we ...

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Vídeo: JoVE Monthly Highlights: November 2017

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Um ano em análise

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...