A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

0:38

Plasmid DNA Linearization

1:26

Full-Length Hepatitis E Virus (HEV) Genotype 3 p6 DNA In Vitro Transcription and RNA Purification

2:35

HepG2 Cell Preparation

3:47

HepG2 Cell Electroporation

5:40

Intra- and Extracellular Cell Culture Derived HEV (HEVcc) Particle Harvest

7:10

HepG2/C3A Cell Infection

8:47

Results: Representative High Titer HEV Stock Preparation and Characterization

9:46

Conclusion