Transient Transfection of the Expression Plasmids into the Cells
2:06
Measuring Luminescence
2:57
Results: Split Luciferase Complementation Assay Performed in HEK293T
4:46
Conclusion
Transcrição
Our protocol allows to identify and analyze interactions between multi transmembrane proteins of the endoplasmic reticulum and golgi complex in living cells at low expression levels. The main advantage of this technique is it's to special sensitiv
Faça login ou inicie seu teste gratuito para acessar este conteúdo
The protocol presented here is intended to demonstrate the occurrence of heterologous interactions between Golgi-resident type III membrane proteins with cytoplasmically exposed N- and/or C-termini in live mammalian cells using the most recent variant of the split luciferase complementation assay.