March 20th, 2021
•A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.
Tags
Vídeos Relacionados
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados