Device Pre‐Assembly, Sample Loading, and Device Assembly
2:36
Device Storage, Crystallization Optimization, and Calibration
3:42
Optical Scanning
4:59
X‐Ray Diffraction
5:34
Results: On‐Chip Protein Crystallization Platform for Automated Serial Diffraction Collection
6:45
Conclusion
TRANSCRIÇÃO
This protocol demonstrate protein crystallization on a chip-like device in x-ray diffraction data recollection. This device is called a crystal-on-crystal because protein crystals grow on a single quartz crystal. Upon successful growth of protein
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This contribution describes how to set up protein crystallization on crystal-on-crystal devices and how to perform automated serial data collection at room temperature using the on-chip crystallization platform.