II. RNA‐seq differential expression analysis through “limma”
8:53
III. RNA‐seq differential expression analysis through “edgeR”
11:34
IV. RNA‐seq differential expression analysis through “DESeq2”
13:51
V. Venn diagram
14:36
Conclusion
Transcrição
Three differential expression analysis methods for RNA sequencing:limma, EdgeR, and DESeq2. Open the RStudio program and then load R file, DEGs. The file can be acquired from supplementary files.One.
Downloading and pre-processing of data.1.
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A detailed protocol of differential expression analysis methods for RNA sequencing was provided: limma, EdgeR, DESeq2.