Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

3.4K Views

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12:44 min

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January 27th, 2023

DOI :

10.3791/64186-v

January 27th, 2023

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3,368 Views

Meagan J. Makarcyzk^1,2, Zhong Alan Li^1,3, Ilhan Yu¹, Haruyo Yagi¹, Xiurui Zhang¹, Lauren Yocum¹, Eileen Li¹, Madalyn R. Fritch¹, Qi Gao⁴, Bruce A. Bunnell⁵, Stuart B. Goodman^4,6, Rocky S. Tuan^1,8, Peter G. Alexander^1,7, Hang Lin^1,2,7

¹Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, ²Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, ³Department of Neurobiology, University of Pittsburgh School of Medicine, ⁴Department of Orthopaedic Surgery, Stanford University, ⁵Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, ⁶Department of Bioengineering, Stanford University, ⁷McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, ⁸The Chinese University of Hong Kong

Capítulos

0:05

Introduction

0:43

Engineering the Tissue Components

5:49

Establishing the Minijoint Chip

8:13

Individual Tissue Collection

9:35

Results: Establishment of a Miniature Synovial Joint-Mimicking Microphysiological System

12:07

Conclusion

Transcrição

Our ability to accurately predict how humans respond to drugs during clinical trials is limited due to the lack of physiologically relevant preclinical models. This microphysiological system can address that disparity. This human cell derived syst

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Resumo

We provide detailed methods for generating four types of tissues from human mesenchymal stem cells, which are used to recapitulate the cartilage, bone, fat pad, and synovium in the human knee joint. These four tissues are integrated into a customized bioreactor and connected through microfluidics, thus generating a knee joint-on-a-chip.

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Keywords Knee Joint on a chip

Microphysiological System

Osteoarthritis

Disease Modifying Osteoarthritis Drugs

GelMA

Methacrylated Gelatin

Mesenchymal Stem Cells

3D Printed Bioreactor

Preclinical Model

Drug Testing