expression-recombinant-cellulase-cel5a-from-trichoderma-reesei

Expression of Recombinant Cellulase Cel5A from <em>Trichoderma reesei</em> in Tobacco Plants

Cellulose degrading enzymes, cellulases, are targets of both research and industrial interests. The preponderance of these enzymes in difficult-to-culture ...

RWTH Aachen University

There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually conducted in batch mode without active pH-control, but with high buffer concentrations to prevent excessive pH-drifts. These differences make it difficult to compare results from screening experiments and laboratory and technical scale cultivations and, thus, complicate rational process development. In particular, the pH-value plays an important role in fermentation processes due to the narrow physiological or optimal pH-range of microorganisms. To reduce the differences between the scales and to establish a pH-control in shake flasks, a newly developed easy to use polymer-based controlled-release system is presented in this paper. This system consists of bio-compatible silicone discs embedding the alkaline reagent Na2CO3. Since the sodium carbonate is gradually released from the discs in pre-determined kinetics, it will ultimately compensate the decrease in pH caused by the biological activity of microorganisms.

Johannes Klinger

Institute for Molecular Biotechnology,
Bio7,
Institute for Molecular Biotechnology, Bio7

RWTH Aachen University

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Johannes Klinger

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Institute for Molecular Biotechnology,Bio7,Institute for Molecular Biotechnology, Bio7

RWTH Aachen University

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Institute for Molecular Biotechnology,
Bio7,
Institute for Molecular Biotechnology, Bio7