Joshua W. Shaevitz

Department of Physics,
Lewis-Sigler Institute for Integrative Genomics,
Department of Physics, Lewis-Sigler Institute for Integrative Genomics

Princeton University

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PUBLICATIONS

An automated two-dimensional optical force clamp for single molecule studies.

Biophysical journal Jul, 2002 | Pubmed ID: 12080136

Probing the kinesin reaction cycle with a 2D optical force clamp.

Proceedings of the National Academy of Sciences of the United States of America Mar, 2003 | Pubmed ID: 12591957

Backtracking by single RNA polymerase molecules observed at near-base-pair resolution.

Nature Dec, 2003 | Pubmed ID: 14634670

Statistical kinetics of macromolecular dynamics.

Biophysical journal Oct, 2005 | Pubmed ID: 16040752

Spiroplasma swim by a processive change in body helicity.

Cell Sep, 2005 | Pubmed ID: 16179261

Picocalorimetry of transcription by RNA polymerase.

Biophysical journal Dec, 2005 | Pubmed ID: 16239336

Direct observation of base-pair stepping by RNA polymerase.

Nature Nov, 2005 | Pubmed ID: 16284617

Evidence that focal adhesion complexes power bacterial gliding motility.

Science (New York, N.Y.) Feb, 2007 | Pubmed ID: 17289998

Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function.

Journal of the Optical Society of America. A, Optics, image science, and vision Sep, 2007 | Pubmed ID: 17767232

Load fluctuations drive actin network growth.

Proceedings of the National Academy of Sciences of the United States of America Oct, 2007 | Pubmed ID: 17895390

Super-resolution for a 3D world.

Nature methods Jun, 2008 | Pubmed ID: 18511914

Curvature and torsion in growing actin networks.

Physical biology , 2008 | Pubmed ID: 18560043

Slow stress propagation in adherent cells.

Biophysical journal Dec, 2008 | Pubmed ID: 18805929

Active and passive mechanisms of intracellular transport and localization in bacteria.

Current opinion in microbiology Dec, 2008 | Pubmed ID: 19007909

Effect of aberration on height calibration in three-dimensional localization-based microscopy and particle tracking.

Applied optics Apr, 2009 | Pubmed ID: 19340142

Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria.

Proceedings of the National Academy of Sciences of the United States of America May, 2010 | Pubmed ID: 20439764

The structure and function of bacterial actin homologs.

Cold Spring Harbor perspectives in biology Sep, 2010 | Pubmed ID: 20630996

Motor-driven intracellular transport powers bacterial gliding motility.

Proceedings of the National Academy of Sciences of the United States of America May, 2011 | Pubmed ID: 21482768

The bacterial actin MreB rotates, and rotation depends on cell-wall assembly.

Proceedings of the National Academy of Sciences of the United States of America Sep, 2011 | Pubmed ID: 21903929

Direct measurement of cell wall stress stiffening and turgor pressure in live bacterial cells.

Physical review letters Oct, 2011 | Pubmed ID: 22107320

INSTITUTIONS

Princeton University

JOVE ARTICLES

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Siyuan Wang¹,

Hugo Arellano-Santoyo²,

Peter A. Combs²,

Joshua W. Shaevitz²

¹Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics, Princeton University,

²Department of Physics, Lewis-Sigler Institute for Integrative Genomics, Princeton University

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Joshua W. Shaevitz

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Physics,Lewis-Sigler Institute for Integrative Genomics,Department of Physics, Lewis-Sigler Institute for Integrative Genomics

Princeton University

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Department of Physics,
Lewis-Sigler Institute for Integrative Genomics,
Department of Physics, Lewis-Sigler Institute for Integrative Genomics