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The Complete and Updated "Rotifer Polyculture Method" for Rearing First Feeding Zebrafish

The zebrafish (Danio rerio) is a model organism of increasing importance in many fields of science.  One of the most demanding technical aspects of ...

Boston Children's Hospital

The zebrafish and the medaka are both important model organisms in biomedical research. Both species are frequently characterized as having a generation time of approximately 2-4 months, but the precise onset of sexual maturity and the variability of reproductive success with age have not been previously examined. The authors studied reproduction in replicate groups of wild-type zebrafish (strain AB) and medakas (strain Cab) that were maintained together in the same aquaculture system. Length, weight and survival of the fish were measured and recorded once per week. Reproductive success and viability of offspring were also evaluated. Both zebrafish and medakas began producing viable embryos within 60 d post-fertilization. These findings show that it is possible to successfully maintain populations of both species within the same research infrastructure without compromising reproductive success or embryo viability.

Kara Maloney

Aquatic Resources Program

Boston Children's Hospital

The Complete and Updated "Rotifer Polyculture Method" for Rearing First Feeding Zebrafish

Kara Maloney

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Boston Children's Hospital

The Complete and Updated "Rotifer Polyculture Method" for Rearing First Feeding Zebrafish

Aquatic Resources Program