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David Gonzalez-Perez

Department of Biocatalysis

Institute of Catalysis, CSIC

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PUBLICATIONS

Directed evolution of a temperature-, peroxide- and alkaline pH-tolerant versatile peroxidase.

The Biochemical journal Jan, 2012 | Pubmed ID: 21980920

Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics.

The Journal of biological chemistry Jun, 2012 | Pubmed ID: 22511773

Saccharomyces cerevisiae in directed evolution: An efficient tool to improve enzymes.

Bioengineered bugs May-Jun, 2012 | Pubmed ID: 22572788

Blood tolerant laccase by directed evolution.

Chemistry & biology Feb, 2013 | Pubmed ID: 23438751

Functional expression of a blood tolerant laccase in Pichia pastoris.

BMC biotechnology , 2013 | Pubmed ID: 23627343

Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.

PloS one , 2014 | Pubmed ID: 24614282

Directed evolution of unspecific peroxygenase from Agrocybe aegerita.

Applied and environmental microbiology Jun, 2014 | Pubmed ID: 24682297

Bioelectrochemical oxidation of water.

Journal of the American Chemical Society Apr, 2014 | Pubmed ID: 24725275

Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae.

Bioengineered Jul-Aug, 2014 | Pubmed ID: 24830983

Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.

Applied and environmental microbiology Sep, 2015 | Pubmed ID: 26162870

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Institute of Catalysis, CSIC

JOVE ARTICLES

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Javier Viña-Gonzalez¹,

David Gonzalez-Perez¹,

Miguel Alcalde¹

¹Department of Biocatalysis, Institute of Catalysis, CSIC

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David Gonzalez-Perez

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Biocatalysis

Institute of Catalysis, CSIC

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Department of Biocatalysis