John LaCava

RNA degradation by the exosome is promoted by a nuclear polyadenylation complex.

RNA-quality control by the exosome.

Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.

Improved native isolation of endogenous Protein A-tagged protein complexes.

Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition.

The human cap-binding complex is functionally connected to the nuclear RNA exosome.

Expression and detection of LINE-1 ORF-encoded proteins.

Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives.

Rapid, optimized interactomic screening.

Characterization of L1-Ribonucleoprotein Particles.

Optimized Affinity Capture of Yeast Protein Complexes.

Native Elution of Yeast Protein Complexes Obtained by Affinity Capture.

Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture.

Protein Complex Purification by Affinity Capture.

Purification and analysis of endogenous human RNA exosome complexes.