a-new-method-for-qualitative-multi-scale-analysis-bacterial-biofilms

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Bacterial biofilms frequently form on fungal surfaces and can be involved in numerous bacterial-fungal interaction processes, such as metabolic ...

INRA Université de Lorraine

Effects of traffic-related nitrogenous emissions on purple moor grass (Molinia caerulea (L.) Moench) transplants, used here as a new biomonitoring species, were assessed along 500 m long transects orthogonal to roads located in two open areas in the Maurienne valley (French Alps). Leaves were sampled during summer 2004 and 2005 for total N-content and (15)N-abundance determination while nitrogen oxides (NO and NO(2)) concentrations were determined using passive diffusion samplers. A significant and negative correlation was observed between plant total N-content, and (15)N-abundance and the logarithm of the distance to the road axis. The strongest decreases in plant N parameters were observed between 15 and 100 m from road axis. They were equivalent to background levels at a distance of about 800 m from the roads. In addition, motor vehicle pollution significantly affected vegetation at road edge, as was established from the relationship between leaf (15)N-abundance, total N-content and road traffic densities.

Biomonitoring of traffic-related nitrogen oxides in the Maurienne valley (Savoie, France), using purple moor grass growth parameters and leaf (15)N/(14)N ratio.

Liming is a forest practice used to counteract forest decline induced by soil acidification. It consists of direct Ca and Mg input in forest soil and restores tree mineral nutrition, but also causes drastic changes in nutrient availability in soil. Ectomycorrhizal (ECM) fungi significantly contribute in nutrient uptake by trees, and can recover them through organic acid secretion or through enzymatic degradation of organic matter. The symbiotic fungi use their extraradical mycelium for nutrient uptake, and then store them into the ECM mantle. In this study we measured how liming influences element contents in the mantle of Lactarius subdulcis ECMs, an abundant and particularly active in oxalate and laccase secretion in beech stands. For this purpose we used SEM observation coupled with energy- (EDX) and wavelength-dispersive-X-ray microanalyses (WDX). Results showed that ECM mantles of this species presented significantly higher Ca, Mg, Mn, K, Si, Al and Fe contents in limed plots. The nutrient amounts of L. subdulcis ECMs were significantly different between individuals for all the elements, showing a differential storage ability between individuals. The storage role of the ECM mantle can be interpreted in two different ways: i) a detoxification role for Al or heavy metals and ii) an increased potential nutrient resource by the fungus, which can benefit the tree.

Liming in a beech forest results in more mineral elements stored in the mantle of Lactarius subdulcis ectomycorrhizas.

Plant water loss and CO2 uptake are controlled by valve-like structures on the leaf surface known as stomata. Stomatal aperture is regulated by hormonal and environmental signals. We show here that stomatal sensitivity to the drought hormone abscisic acid (ABA) is acquired during leaf development by exposure to an increasingly dryer atmosphere in the rosette plant Arabidopsis. Young leaves, which develop in the center of the rosette, do not close in response to ABA. As the leaves increase in size, they are naturally exposed to increasingly dry air as a consequence of the spatial arrangement of the leaves, and this triggers the acquisition of ABA sensitivity. Interestingly, stomatal ABA sensitivity in young leaves is rapidly restored upon water stress. These findings shed new light on how plant architecture and stomatal physiology have coevolved to optimize carbon gain against water loss in stressing environments.

Developmental priming of stomatal sensitivity to abscisic acid by leaf microclimate.

Chemosynthetic mats involved in cycling sulfur compounds are often found in hydrothermal vents, cold seeps and whale falls. However, there are only few records of wood fall mats, even though the presence of hydrogen sulfide at the wood surface should create a perfect niche for sulfide-oxidizing bacteria. Here we report the growth of microbial mats on wood incubated under conditions that simulate the Mediterranean deep-sea temperature and darkness. We used amplicon and metagenomic sequencing combined with fluorescence in situ hybridization to test whether a microbial succession occurs during mat formation and whether the wood fall mats present chemosynthetic features. We show that the wood surface was first colonized by sulfide-oxidizing bacteria belonging to the Arcobacter genus after only 30 days of immersion. Subsequently, the number of sulfate reducers increased and the dominant Arcobacter phylotype changed. The ecological succession was reflected by a change in the metabolic potential of the community from chemolithoheterotrophs to potential chemolithoautotrophs. Our work provides clear evidence for the chemosynthetic nature of wood fall ecosystems and demonstrates the utility to develop experimental incubation in the laboratory to study deep-sea chemosynthetic mats.

Christophe Rose

Ecologie et Ecophysiologie Forestières - PTEF,
UMR 1137,
Ecologie et Ecophysiologie Forestières - PTEF, UMR 1137

INRA Université de Lorraine

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Christophe Rose

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Ecologie et Ecophysiologie Forestières - PTEF,UMR 1137,Ecologie et Ecophysiologie Forestières - PTEF, UMR 1137

INRA Université de Lorraine

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Ecologie et Ecophysiologie Forestières - PTEF,
UMR 1137,
Ecologie et Ecophysiologie Forestières - PTEF, UMR 1137