behavioral-assessments-spontaneous-locomotion-murine-mptp-induced

Behavioral Assessments of Spontaneous Locomotion in a Murine MPTP-induced Parkinson's Disease Model

Parkinson&#8217;s disease (PD) is a common neurodegenerative disorder disease, causing the phenomenon of shaking, rigidity, slowness of movement and ...

Nankai University

With increased social pressure, individuals face a high risk of depression. Subsequently, depression affects cognitive behaviour and negatively impacts daily life. Fortunately, the Traditional Chinese Medicine Jia Wei Xiao Yao (JWXY) capsule is effective in reducing depression and improving cognitive behaviour.

Reversal of reserpine-induced depression and cognitive disorder in zebrafish by sertraline and Traditional Chinese Medicine (TCM).

As an excellent model organism, zebrafish have been widely applied in many fields. The accurate identification and tracking of individuals are crucial for zebrafish shoaling behaviour analysis. However, multi-zebrafish tracking still faces many challenges. It is difficult to keep identified for a long time due to fish overlapping caused by the crossings. Here we proposed an improved Histogram of Oriented Gradient (HOG) algorithm to calculate the stable back texture feature map of zebrafish, then tracked multi-zebrafish in a fully automated fashion with low sample size, high tracking accuracy and wide applicability. The performance of the tracking algorithm was evaluated in 11 videos with different numbers and different sizes of zebrafish. In the Right-tailed hypothesis test of Wilcoxon, our method performed better than idTracker, with significant higher tracking accuracy. Throughout the video of 16 zebrafish, the training sample of each fish had only 200-500 image samples, one-fifth of the idTracker's sample size. Furthermore, we applied the tracking algorithm to analyse the depression and hypoactivity behaviour of zebrafish shoaling. We achieved correct identification of depressed zebrafish among the fish shoal based on the accurate tracking results that could not be identified by a human.

Automatic multiple zebrafish tracking based on improved HOG features.

Deltamethrin is widely used because of its low toxicity and high efficiency. Although its potential toxicity has been reported, its effects on cardiovascular system and motor behavior and its underlying mechanisms have remained unclear. In this study, the effects of deltamethrin on the development, cardiovascular system and motor behavior of zebrafish larvae and their possible mechanisms were evaluated using the transgenic zebrafish Tg (kdrl:mCherry) and Tg(myl7:GFP). At 72 hpf, the body length of larvae shortened, the head and eye area decreased, and the hatching rate increased. Acridine orange staining showed that treated zebrafish larvae produced different degrees of apoptosis in the head, body, heart and tail regions. Quantitative fluorescence intensity showed a dose-dependent increase in apoptosis signal, indicating that deltamethrin could induce apoptosis. Confocal images and fluorescence intensity quantification of red fluorescent protein-labeled vascular endothelial cell and green fluorescent protein-labeled transgenic zebrafish more clearly reflected the dose-dependent cardiac and vascular morphology and the damage caused by deltamethrin. Deltamethrin significantly induced vascular endothelial growth factor flk1 and fli-1, cardiac development-related gene myl7 decreased in a dose-dependent manner. In addition, deltamethrin increased the thigmotaxis of zebrafish larvae, causing anxiety-like behavior. Our study showed that deltamethrin could cause developmental toxicity, apoptosis, cardiovascular system damage and anxiety-like behavior, which provided a reference for the use of deltamethrin in agricultural production.

Xi-Zeng Feng

State Key Laboratory of Medicinal Chemical Biology,
The Key Laboratory of Bioactive Materials,
Ministry of Education,
College of Life Science,
State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science

Nankai University

Behavioral Assessments of Spontaneous Locomotion in a Murine MPTP-induced Parkinson's Disease Model