native-cell-membrane-nanoparticles-system-for-membrane-protein

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Protein-protein interactions in cell membrane systems play crucial roles in a wide range of biological processes- from cell-to-cell interactions to signal ...

Virginia Commonwealth University

Analyses of the hydropathic environments of protein amino acid residues reveal structural information on multiple levels. The interactions made by each residue are the basis for sidechain (rotamer) conformation and ultimately for secondary, tertiary and even quaternary protein structure. By identifying and characterizing the interactions for each residue type, we are developing a basis set of environmental data that can be used to understand protein structure. This work focuses alanine and its roles. We calculated and analyzed separately backbone-to-environment and sidechain-to-environment 3D maps for over 57,000 alanines that, with respect to hydrophobic and polar interactions, show the environment around each. After binning by backbone ϕ and ψ angles, we clustered each bin with k-means based on calculated map similarities between map-map pairs. Four bins were examined in detail: one in the β-pleat region, two in the right-hand α-helix (RHα) region and one in the left-hand α-helix region of the Ramachandran plot. All regions indicated a common map motif of hydrophobic-hydrophobic interactions along the CA-CB axis, accounting for 62% in the β-pleat bin, about one-third in the two RHα bins and 42% in the LHα bin. Another shared motif shows no interactions along the CA-CB axis; this was uncommon (8%) in β-pleat, but >30% elsewhere. The maps calculated for the two RHα bins are extremely similar (pairwise >0.9787), which suggests that the hydropathic interaction sets or motifs found around each residue are conserved. Altogether, these results are integral to a new paradigm for understanding protein structure and function.

Claudio Catalano

Department of Medicinal Chemistry,
School of Pharmacy,
Institute for Structural Biology,
Drug Discovery and Development,
Department of Medicinal Chemistry, School of Pharmacy,
Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy

Virginia Commonwealth University

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis