an-efficient-method-for-directed-hepatocyte-like-cell-induction-from

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

The potential functions of hepatocyte-like cells (HLCs) derived from human embryonic stem cells (hESCs) hold great promise for disease modeling and drug ...

Shantou University Medical College

Hepatitis B virus (HBV) infection may affect sperm motility in patients with HBV. HBV surface protein (HBs) decreases mitochondrial membrane potential, impairs motility and induces apoptotic-like changes in human spermatozoa. However, little is known about how human spermatozoa respond to reactive oxygen species (ROS; mainly peroxides) induced by HBs. In this study, HBs induced supraphysiological ROS levels in human spermatozoa and reduced the formation of 2-cell embryos (obtained from hamster oocytes and human spermatozoa). HBs induced a pre-apoptotic status in human spermatozoa, as well as antioxidant defences by increasing glutathione peroxidase 4 (GPX4) and peroxiredoxin 5 (PRDX5) levels. These results highlight the molecular mechanism responsible for the oxidative stress in human spermatozoa exposed to HBV and the antioxidant defence response involving GPX4 and PRDX5.

Xiaoling Xie

Stem Cell Research Center,
The Center for Reproductive Medicine,
Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology

Shantou University Medical College

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Xiaoling Xie

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Stem Cell Research Center,The Center for Reproductive Medicine,Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology

Shantou University Medical College

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Stem Cell Research Center,
The Center for Reproductive Medicine,
Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology