fabrication-use-dry-macroporous-alginate-scaffolds-for-viral

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Genetic engineering of T cells for CAR-T cell therapy has come to the forefront of cancer treatment over the last few years. CAR-T cells are produced by ...

University of North Carolina at Chapel Hill and North Carolina State University

North Carolina State University

Despite their clinical success, chimeric antigen receptor (CAR)-T cell therapies for B cell malignancies are limited by lengthy, costly and labor-intensive ex vivo manufacturing procedures that might lead to cell products with heterogeneous composition. Here we describe an implantable Multifunctional Alginate Scaffold for T Cell Engineering and Release (MASTER) that streamlines in vivo CAR-T cell manufacturing and reduces processing time to a single day. When seeded with human peripheral blood mononuclear cells and CD19-encoding retroviral particles, MASTER provides the appropriate interface for viral vector-mediated gene transfer and, after subcutaneous implantation, mediates the release of functional CAR-T cells in mice. We further demonstrate that in vivo-generated CAR-T cells enter the bloodstream and control distal tumor growth in a mouse xenograft model of lymphoma, showing greater persistence than conventional CAR-T cells. MASTER promises to transform CAR-T cell therapy by fast-tracking manufacture and potentially reducing the complexity and resources needed for provision of this type of therapy.

Pritha Agarwalla

Joint Department of Biomedical Engineering

University of North Carolina at Chapel Hill and North Carolina State University

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Pritha Agarwalla

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Joint Department of Biomedical Engineering

University of North Carolina at Chapel Hill and North Carolina State University

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Joint Department of Biomedical Engineering