standardized-identification-compound-structure-tibetan-medicine-using

Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

Tibetan medicines are complex and contain numerous unknown compounds, making in-depth research on their molecular structures crucial. Liquid ...

visualization-metabolites-identified-spatial-metabolome-traditional

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

The medicinal use of traditional Chinese medicine is mainly due to its secondary metabolites. Visualization of the distribution of these metabolites has ...

Chengdu University of Traditional Chinese Medicine

The study was to investigate the effect and action mechanism of aconitine and emodin on the function of the interstitial cells of Cajal (ICC) cultured in vitro. ICC cells were treated with aconitine (0.05-8%) and emodin (0.001-2%) in single or combined synchronous experiments and the effect of emodin on aconitine was evaluated using cell viability as end-point. Both the two compounds had toxicity on ICC cell. The cell membrane integrity impairment caused by the exposure lead to the efflux of intracellular ionic ([Na+], [Ca2+] and [K+]) and the deactivation of the Na+-K+-ATPase. The ionic disturbance caused the interruption of the cellular breathing chain and resulted in anaerobic metabolism increase and the glycogen massive decomposition, at last the energy metabolism in the cells was obstructed. But the antagonist effect existed when the two compounds were exposed to ICC cells together. The compatibility (aonitine:emodin as 2:1), can significantly reversed the toxicity of aconition. In addition, synergistic effects were never observed in the range of concentrations considered. Although emodin can defer the aconition's toxicity on ICC cell, the impairment can't be totally inhibited by the compatibility with time went on. The results of our work represent a starting point to generate novel information on the interactions between aconitine and emodin in vitro, as well as a new relevant experimental approach useful to investigate the Herb compatibility with aconite and rhubarb and reference for the clinic.

The toxicity of aconitine, emodin on ICC cell and the antagonist effect of the compatibility.

According to folk usage of Aconitum carmichaelii Debx., the present study was designed to determine the feasibility of the stems and leaves of Aconitum carmichaelii Debx. as a new medicinal resource. Fourteen alkaloids in mother roots, fibrous roots, stems, and leaves of Aconitum carmichaelii Debx. were measured by HPLC-MS/MS. And multivariate analysis methods, such as clustering analysis and principal component analysis, were applied to analyze the difference among various parts. In addition, the acute toxicity, analgesia, and anti-inflammatory tests were carried out. The results suggested that the contents of alkaloids in mother roots and fibrous roots were approximate, but those of leaves and stems were different from mother roots and fibrous roots. The results of the acute toxicity testing demonstrated the toxicity of fibrous root was strongest, and mother roots were slightly less toxic than fibrous roots. The stems and leaves were far less toxic than mother and fibrous roots. In addition, the analgesia and inflammatory tests showed the effects of the various tissues had no difference each other. These results provided a basis for developing new complementary and alternative treatments for rheumatoid arthritis patients. Simultaneously, the approach may also turn wastes into treasure and promote the development of circular economy.

Stems and leaves of Aconitum carmichaelii Debx. as potential herbal resources for treating rheumatoid arthritis: Chemical analysis, toxicity and activity evaluation.

Yuan Pan

Innovative Institute of Chinese Medicine and Pharmacy,
State Key Laboratory of Southwestern Chinese Medicine Resources,
State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy

Chengdu University of Traditional Chinese Medicine

Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI