co-culture-transduction-murine-thymocytes-on-delta-like-4-expressing

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Transduced mouse immature thymocytes can be differentiated into T cells&#160;in vitro&#160;using the delta-like 4-expressing bone marrow stromal cell line ...

National Institutes of Health

The IL-7 pathway is required for normal T cell development and survival. In recent years the pathway has been shown to be a major driver of acute lymphoblastic leukemia (ALL), the most common cancer in children. Gain-of-function mutations in the alpha chain of the IL-7 receptor found in ALL patients clearly demonstrated that this pathway was a driver. However mutant IL-7R alone was insufficient to transform primary T cell progenitors, indicating that cooperating mutations were required. Here we review evidence for additional oncogenic mutations in the IL-7 pathway. We discuss several oncogenes, loss of tumor suppressor genes and epigenetic effects that can cooperate with mutant IL-7 receptor. These include NRas, HOXA, TLX3, Notch 1, Arf, PHF6, WT1, PRC, PTPN2 and CK2. As new therapeutics targeting the IL-7 pathway are developed, combination with agents directed to cooperating pathways offer hope for novel therapies for ALL.

Hila Y. Winer

Cytokines and Immunity Section,
Cancer Innovation Laboratory,
National Cancer Institute,
Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute

National Institutes of Health

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Hila Y. Winer

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Cytokines and Immunity Section,Cancer Innovation Laboratory,National Cancer Institute,Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute

National Institutes of Health

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Cytokines and Immunity Section,
Cancer Innovation Laboratory,
National Cancer Institute,
Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute