in-vivo-visualization-spontaneous-activity-neonatal-mouse-sensory

In Vivo Visualization of Spontaneous Activity in Neonatal Mouse Sensory Cortex at a Single-Neuron Resolution

The mammalian brain undergoes dynamic developmental changes at both the cellular and circuit levels throughout prenatal and postnatal periods. Following ...

Kumamoto University

In vivo calcium imaging is essential to elucidate unique synchronous activities observed in the developing brain. Here, we present a protocol to image and analyze activity patterns in neonatal mouse neocortex in a single-cell level. We describe steps for in utero electroporation, cranial window surgery, two-photon imaging, and activity correlation analysis. This protocol facilitates the understanding of neuronal activities and activity-dependent circuit formation during development. For complete details on the use and execution of this protocol, please refer to Mizuno et al. (2014), Mizuno et al. (2018a), and Mizuno et al. (2018b)..

Takamitsu Egashira

Laboratory of Multi-Dimensional Imaging,
International Research Center for Medical Sciences (IRCMS),
Graduate School of Medical Sciences,
Laboratory of Multi-Dimensional Imaging, International Research Center for Medical Sciences (IRCMS)

Kumamoto University

In Vivo Visualization of Spontaneous Activity in Neonatal Mouse Sensory Cortex at a Single-Neuron Resolution